| Two Fully Referenced Alleles |
|
Exon 2 |
|
Exon 3 |
|
Exon 4 |
|
|
|
Exon 2 |
|
Exon 3 |
|
Exon 4 |
|
|
| full | 270 | + | 276 | + | 276 | = | 822 |
| part | 0 | + | 0 | + | 0 | = | 0 |
| unreferenced | 0 | + | 0 | + | 0 | = | 0 |
| One Partially Referenced Allele |
|
Exon 2 |
|
Exon 3 |
|
Exon 4 |
|
|
|
Exon 2 |
|
Exon 3 |
|
Exon 4 |
|
|
| full | 270 | + | 276 | + | 0 | = |
546 |
| part | 0 | + | 0 | + | 276 | = |
276 |
| unreferenced | 0 | + | 0 | + | 0 | = | 0 |
| Two Partially Referenced Alleles |
|
Exon 2 |
|
Exon 3 |
|
Exon 4 |
|
|
|
Exon 2 |
|
Exon 3 |
|
Exon 4 |
|
|
| full | 270 | + | 276 | + | 0 | = | 546 |
| part | 0 | + | 0 | + | 0 | = | 0 |
| unreferenced | 0 | + | 0 | + |
276 | = | 276 |
| Matching with Partially Referenced Alleles |
| The observed sequence covers exons 2, 3 and 4. |
| Observed |
Exon 2 |
|
Exon 3 |
|
Y |
|
|
| There are four alleles in the library that match the observed sequence in exons 2 and 3. |
| Allele X*01 |
Exon 2 |
|
Exon 3 |
|
C |
|
|
| Allele X*02 |
Exon 2 |
|
Exon 3 |
|
G |
|
|
| Allele X*03 |
Exon 2 |
|
Exon 3 |
|
? |
|
|
| Allele X*04 |
Exon 2 |
|
Exon 3 |
|
? |
|
|
| We can reject some of the combinations by looking at the exon 4 sequence. |
| X*01 + X*02 |
Exon 2 |
|
Exon 3 |
|
C + G = S |
|
|
| This combination will give C + G = S in Exon 4 so we can reject it because the observed sequence is Y. |
| X*01 + X*03 |
Exon 2 |
|
Exon 3 |
|
C + ? = C, S, or Y |
|
|
| This combination might give C, S or Y in Exon 4. We cannot reject it because it might be a match. |
| X*02 + X*03 |
Exon 2 |
|
Exon 3 |
|
G + ? = G, S, or K |
|
|
| This combination might give G, S or K in Exon 4. We can reject it because it cannot give a Y. |
| X*03 + X*04 |
Exon 2 |
|
Exon 3 |
|
? |
|
|
| We don't know anything about exon 4. We cannot reject this allele pair because it might give a Y. |